Journal: Metabolites

Article Title: Altered Pulmonary Glucose Transport Is Restored by Metformin Treatment in an Obese Type 2 Diabetic Animal Model

doi: 10.3390/metabo15110717

Figure Lengend Snippet: Type 2 diabetes (T2Dx) induced decreased pulmonary protein expression of the class I glucose transporter (GLUT), which was partially rescued by in vivo metformin (Met) treatment. Total protein expression of ( A ) GLUT1, ( B ) GLUT2, and ( C ) GLUT4 in the whole lung of control (con), untreated T2Dx, and metformin-treated T2Dx mice. Top panels: representative Western blot from total lysate of the whole lung; loading control: beta actin. Bottom panels: Mean ± SE of total GLUT protein content (values normalized to β-actin and its respective controls, n = 4–6/group). * p < 0.05 vs. control. Methods: Western blotting.

Article Snippet: Primary antibodies used included: polyclonal rabbit anti-mouse GLUT1 (1:500, Abcam #ab652), GLUT2 (1:500, Santa Cruz #sc-7582), GLUT3 (1:500, Abcam #ab54460), GLUT4 (1:750, AbD Serotec #4670-1704), GLUT8 (1:500, Bioss #bs4241R), GLUT10 (1:750, Thermo Fisher #PA1-46137), and GLUT12 (1:500, Abcam #ab75441). β-actin was detected using a mouse monoclonal antibody (Santa Cruz #sc-47778).

Techniques: Expressing, In Vivo, Control, Western Blot

Journal: Metabolites

Article Title: Altered Pulmonary Glucose Transport Is Restored by Metformin Treatment in an Obese Type 2 Diabetic Animal Model

doi: 10.3390/metabo15110717

Figure Lengend Snippet: Type 2 diabetes (T2Dx) decreased pulmonary cell surface glucose transporter (GLUT) protein expression, which was rescued by in vivo metformin (Met) treatment. Cell-surface protein expression of ( A ) GLUT4 and ( B ) GLUT8 in whole lung of control (Con), untreated T2Dx, and metformin-treated T2Dx animals. Top panels: representative Western blot. Bottom panels: Mean ± SE of cell surface GLUT protein content (values normalized to respective controls, n = 4/group). L: Labeled (cell surface fraction), UL: unlabeled (intracellular fraction). * p < 0.05 vs. control, via one-way ANOVA. Methods: biotinylated photolabeling assay.

Article Snippet: Primary antibodies used included: polyclonal rabbit anti-mouse GLUT1 (1:500, Abcam #ab652), GLUT2 (1:500, Santa Cruz #sc-7582), GLUT3 (1:500, Abcam #ab54460), GLUT4 (1:750, AbD Serotec #4670-1704), GLUT8 (1:500, Bioss #bs4241R), GLUT10 (1:750, Thermo Fisher #PA1-46137), and GLUT12 (1:500, Abcam #ab75441). β-actin was detected using a mouse monoclonal antibody (Santa Cruz #sc-47778).

Techniques: Expressing, In Vivo, Control, Western Blot, Labeling